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目的探讨蛋氨酸胆碱对铅暴露SD大鼠体内微量元素影响。方法将60只SD大鼠随机分为空白对照组、染铅组、蛋氨酸胆碱组、铅+低、高剂量蛋氨酸胆碱组,分别给予铅(饮水)和蛋氨酸胆碱(灌胃),麻醉取脑、肾、肝和骨,采用电感耦合等离子发射光谱法测定组织中铅、铜、铁、锰、锌及钙含量。结果染铅后,脑、肝、肾、骨组织中铅含量明显增高,分别为(0.631±0.188),(1.178±0.354),(12.669±2.682)和(165.621±12.015)μg/g,铅+低、高剂量蛋氨酸胆碱组肝组织铅含量分别降至(0.955±0.299)和(0.637±0.164)μg/g;铅+高剂量蛋氨酸胆碱组肾铅和骨铅含量分别降至(9.789±1.743)和(132.742±17.743)μg/g,明显低于铅对照组(P<0.01);铅对照组脑铁和铜及肝铁含量高于空白对照组,给予蛋氨酸胆碱后,脑铁和铜及肝铁含量下降至空白对照组水平。结论蛋氨酸胆碱可降低肝、肾和骨组织中铅含量,对铜、铁、锰、锌无明显影响。
Objective To investigate the effects of methionine choline on trace elements in lead exposed SD rats. Methods Sixty SD rats were randomly divided into blank control group, lead group, methionine choline group, lead + low and high dose methionine choline group, lead (drinking water) and methionine choline (gavage) The contents of lead, copper, iron, manganese, zinc and calcium in tissues were determined by inductively coupled plasma atomic emission spectrometry. Results Lead (Pb) contents in brain, liver, kidney and bone were significantly increased after lead exposure (0.631 ± 0.188, 1.178 ± 0.354, 12.669 ± 2.682 and 165.621 ± 12.015 μg / g, respectively) The levels of lead and bone lead in the lead and high-dose methionine choline groups decreased to (9.789 ± 0.995 ± 0.299) and (0.637 ± 0.164) μg / g, respectively 1.743) and (132.742 ± 17.743) μg / g, respectively, which were significantly lower than those of the lead control group (P <0.01). The levels of brain iron, copper and liver iron in the lead control group were higher than those in the blank control group. Copper and liver iron levels decreased to the level of the blank control group. Conclusion Methionine choline can reduce the content of lead in liver, kidney and bone tissue, and has no significant effect on copper, iron, manganese and zinc.