论文部分内容阅读
我县自1962年临床诊断出肾综合征出血热(简HFRS)以来,病例逐年增多,疫区逐渐扩大,严重地危害人民群众,为了进一步开展HFRS的防治工作,我们于1984年11月29日起收集标本,1985年1月进行HFRS病毒的分离工作,获得了成功。去年年底在流行区八里乡和后坝乡采集黑线姬鼠及褐家鼠的肺组织,同时采集遵义县医院经临床诊断为HFRS的早期(发病后3天以内)病人血液10ml。经间接荧光免疫染色法(简IFAT),筛选出抗原性强的阳性鼠肺三个,制成混悬液,与发病3日内病人全血七份分别接种Vero-E_6细胞,得到可连续传代的HFRS病毒株9份(9/10)。选其中病人血病毒株B_2,黑线姬鼠病毒株A_(76)和褐家鼠病株R_(93),与确诊的HFRS病人的双份血清和与国内其他
Since the clinical diagnosis of hemorrhagic fever with renal syndrome (HFRS) in our county since 1962, the number of cases has been increasing year by year, and the epidemic area has gradually expanded, seriously endangering the masses. In order to further carry out the prevention and treatment of HFRS, on November 29, 1984 Collecting specimens, January 1985 HFRS virus separation work, was successful. At the end of last year, the lung tissues of Apodemus agrarius and Rattus norvegicus were collected in Balizu and Houba townships of epidemic areas. Meanwhile, 10ml of blood of patients with early diagnosis of HFRS (less than 3 days after onset) was collected from Zunyi Hospital. Three indirect positive immunofluorescence staining (IFAT) was used to screen out three positive murine lungs, which were made into a suspension and inoculated with Vero-E_6 cells separately from the whole blood of seven patients within 3 days of onset. Nine HFRS strains (9/10). Among them, the patient blood strain B_2, the black line Apodemus agarus strain A_ (76) and the brown rat strain R_ (93) were screened with duplicate sera from patients with confirmed HFRS and with other domestic