目的　探讨致痫剂马桑内酯引发体外培养小胶质细胞激活的细胞内机制。方法　应用马桑内酯 ( CL ,2 5μmol/ L )作用于培养的新生大鼠的小胶质细胞 ,免疫细胞化学观察小胶质细胞的形态学改变及其核转录因子 NF-κB的表达情况。结果　 CL刺激后小胶质细胞形态多为阿米巴形活化表现 ;刺激 10 m in内 NF-κB核表达细胞显著增多 ( P<0 .0 1) ,1h达高峰 ( 97.3 7± 1.94) % ,PDTC ( 10 0μmol/ L )可抑制其核表达。结论　致痫剂马桑内酯可快速激活小胶质细胞 ,其激活的细胞内机制可能与核因子 -κB的活化有关 Objective To investigate the intracellular mechanism of epileptogenic agent-induced formation of microglial cells in vitro. Methods The microglial cells of cultured neonatal rats were treated with CL (25μmol / L). The morphological changes of microglia and the expression of nuclear factor-κB (NF-κB) were observed by immunocytochemistry . Results The morphology of microglial cells was mostly activated by amoebic. The expression of nuclear NF-κB increased significantly (P <0.01) in 10 min, and peaked at 1 h (97.3 7 ± 1.94)%, , PDTC (100μmol / L) inhibited its nuclear expression. CONCLUSIONS: The epileptogenic agent, docetaxel, can rapidly activate microglial cells and its activation of intracellular mechanism may be related to the activation of nuclear factor-κB