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作者应用定向克隆的方法，将从日本血吸虫成虫mRNA合成的cDNA片段，重组入噬菌体λgtllSfi－Not的EcoRⅠ和NotⅠ双酶切点之间。所构成的基因表达文库的库容量为3．13×106重组子。经含有IPTG及X－Gal的颜色选择平皿测定，重组效率为100％。应用抗体探针法已从一部分文库中筛选出25个阳性克隆。从阳性克隆的进一步分析以及应用特定基因引物的PCR扩增，目前已分离出4个具疫苗保护潜能的蛋白编码基因，从而表明文库的质量比较满意。
The authors applied directional cloning method, the cDNA fragment synthesized from mRNA of Schistosoma japonicum was recombined into EcoR I and Not I double cleavage points of bacteriophage λgtllSfi-Not. The constructed gene expression library has a capacity of 3.13 × 106 recombinants. The recombination efficiency was 100% as determined by a color-selective plate containing IPTG and X-Gal. Twenty-five positive clones have been screened from a portion of the library using the antibody probe method. From the further analysis of positive clones and the application of PCR amplification of specific gene primers, four protein coding genes with vaccine protective potential have been isolated, indicating that the quality of the library is satisfactory.