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目的观察肝舒胶囊抗鸭体内乙型肝炎病毒(DHBV)的作用。方法以DHBV静脉注射感染雏鸭为模型,于感染后口服肝舒胶囊28 d,每日剂量分别为1.750、0.875、0.437 g·kg-1。采用斑点杂交方法检测鸭血清中的DHBV DNA;用ELISA快速法检测血清中鸭乙型肝炎病毒表面抗原(DHBSAG),检测用药前、后血清中DHBV DNA的滴度改变、DHBSAG的OD值改变情况。结果高、中剂量肝舒胶囊组用药后7～28 d均出现鸭血清中DHBV DNA的滴度明显降低,但在停药7 d后出现DHBV DNA滴度回升现象;肝舒胶囊3个剂量组治疗期间均对血清中DHBSAG有明显的降低作用。对DHBV DNA和DHBSAG的抑制作用均表现出一定的量效关系。结论肝舒胶囊对鸭体内DHBV有明显的抑制作用。
Objective To observe the effect of Gansu Capsule against Hepatitis B virus (DHBV) in duck body. Methods Intravenous injection of DHBV ducklings as a model, after oral administration of liver Shu Capsule 28 d, the daily doses were 1.750,0.875,0.437 g · kg-1. DHBV DNA in duck serum was detected by dot blot hybridization. DHBSAG in serum was detected by ELISA, the titer of DHBV DNA in serum was detected before and after treatment, and the OD value of DHBSAG was changed . Results The titers of DHBV DNA in duck serum were significantly decreased in high and medium dose Gansu capsules group 7 ~ 28 d after treatment, but the DHBV DNA titer rose after 7 d withdrawal. The three dose groups of Gansu capsule During the treatment of serum DHBSAG significantly reduced. Inhibition of DHBV DNA and DHBSAG showed a certain dose-effect relationship. Conclusions Ganshu capsule has obvious inhibitory effect on DHBV in ducks.