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为除去粘连细胞及细胞团对DNA测量的影响,应用小鼠胸腺细胞测量脉冲面积及宽度,在二维点图用圈除去粘控细胞。结果;DNA测量粘连细胞及细胞团严重影响G0/G1及M2+G2期测量,在固定细胞、实体病细胞等易产生粘连情况,细胞样品DNA测量中应用DDM除去粘连细胞的影响。
To remove the effect of adherent cells and cell mass on DNA measurements, mouse thymocytes were used to measure the pulse area and width and the muco-cells were removed in circles on a two-dimensional dot plot. RESULTS: The results of DNA measurement showed that the adhesion cells and cell mass seriously affected the G0 / G1 and M2 + G2 phase measurements. Adhesion was easily observed in fixed cells and somatic cells, and DDM was used to remove the adhesion cells in DNA samples.