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目的:建立高效液相色谱法测定测定栀子金花丸中黄芩苷、大黄素、大黄酚的含量。方法:色谱柱为Diamonsil C18(250 mm×4.6 mm,5μm);流动相为甲醇-0.4%磷酸溶液,采用梯度洗脱;检测波长为254 nm:柱温:室温,流速:1.0 ml.min-1。结果:黄芩苷进样量在0.154～1.540μg范围内与峰面积线性关系良好,r=0.999 6,平均回收率为100.2%,RSD=1.5%(n=9);大黄素进样量在0.071～0.714μg范围内与峰面积线性关系良好,r=1.000 0,平均回收率为99.7%,RSD=1.2%(n=9);大黄酚进样量在0.147～1.472μg范围内与峰面积线性关系良好,r=0.999 8,平均回收率为100.0%,RSD=1.2%(n=9)。结论:该方法简便、准确、重复性好。
Objective: To establish a HPLC method for the determination of baicalin, emodin and chrysophanol in Zhizi Jinhua Wan. METHODS: Diamonsil C18 (250 mm × 4.6 mm, 5 μm) was used as mobile phase. The mobile phase consisted of methanol-0.4% phosphoric acid solution with gradient elution. The detection wavelength was 254 nm. The column temperature was at room temperature and the flow rate was 1.0 ml · min- 1. Results: The linearity of baicalin injection ranged from 0.154 to 1.540 μg with a peak area of 0.999 6, with an average recovery of 100.2% and a RSD of 1.5% (n = 9). The emodin injection volume was 0.071 The linear range of the peak area was ~ 0.714μg, the average recovery was 99.7% and the RSD was 1.2% (n = 9) for the range of 0.147-1.472μg Good correlation, r = 0.999 8, average recovery 100.0%, RSD = 1.2% (n = 9). Conclusion: The method is simple, accurate and reproducible.