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目的:探讨微小RNA-155(microRNA-155,miR-155)反义寡核苷酸(antisense oligonucleotide,ASO)对乳腺癌MDA-MB-157细胞增殖、凋亡及裸鼠移植瘤的作用。方法:设计合成化学修饰的miR-155 ASO,通过LipofectamineTM 2000转染MDA-MB-157细胞,激光共聚焦检测转染率,real-time PCR测定转染后miR-155表达水平的变化,CCK-8法检测细胞增殖抑制率,流式细胞仪检测细胞凋亡率,观察miR-155 ASO对裸鼠成瘤能力的影响,免疫组织化学法检测瘤体组织Caspase-3的表达。结果:激光共聚焦检测转染率达80%以上。转染miR-155 ASO后,miR-155表达明显下降,细胞增殖能力降低,凋亡增加。miR-155 ASO能明显抑制裸鼠移植瘤生长,抑制率为52.98%,并且能显著增加Caspase-3的表达。结论:miR-155 ASO能显著下调miR-155的表达水平,继而抑制乳腺癌MDA-MB-157细胞的增殖,促进其凋亡;并且通过增加靶基因Caspase-3的表达,抑制裸鼠移植瘤的生长,为miR-155作为乳腺癌治疗靶点提供了实验依据。
AIM: To investigate the effects of microRNA-155 (miR-155) antisense oligonucleotide (ASO) on the proliferation and apoptosis of breast cancer MDA-MB-157 cells and the transplanted tumor in nude mice. Methods: The synthetic chemically modified miR-155 ASO was designed and synthesized. The MDA-MB-157 cells were transfected with LipofectamineTM 2000. The transfection efficiency was detected by confocal laser scanning microscope. The expression of miR-155 was detected by real-time PCR. 8 method to detect the inhibition rate of cell proliferation. Flow cytometry was used to detect the apoptosis rate. The effect of miR-155 ASO on the tumorigenicity of nude mice was observed. The expression of Caspase-3 was detected by immunohistochemistry. Results: Laser confocal detection of transfection rate of more than 80%. Transfection of miR-155 ASO, miR-155 expression was significantly decreased, cell proliferation decreased, increased apoptosis. miR-155 ASO significantly inhibited the growth of transplanted xenografts in nude mice with an inhibition rate of 52.98% and significantly increased Caspase-3 expression. Conclusion: miR-155 ASO can significantly down-regulate the expression of miR-155, and then inhibit the proliferation and promote the apoptosis of breast cancer MDA-MB-157 cells; and inhibit the growth of nude mice by increasing the expression of Caspase-3 Of the growth of miR-155 as a therapeutic target for breast cancer provided experimental basis.