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目的:探讨白细胞介素-1β(IL-1β)在溃疡性结肠炎(UC)中的作用,进一步研究清肠栓治疗UC的作用机理。方法:SD大鼠56只随机分为正常组、模型组(空白组)、西药组(SASP组)、中药组(清肠栓组)共4组,每组14只。用TNBs 100 mg.kg-1灌肠建立大鼠溃疡性结肠炎模型,第3天开始,除正常组外,各组分别给予生理盐水、柳氮磺胺吡啶、清肠栓处理,至7天后处死动物。腹主动脉取血制备血清,采用ELISA法测定各组血清IL-1β的含量。结果:UC大鼠血清IL-1β含量显著升高,西药组及中药组均能降低其含量。中药组与西药组相比,P<0.01。结论:中药清肠栓可以通过降低血清IL-1β含量,调节肠道免疫,达到缓解UC的治疗目的。
Objective: To investigate the role of interleukin-1β (IL-1β) in ulcerative colitis (UC) and further study the mechanism of Qingchang suppository in the treatment of UC. METHODS: Fifty-six SD rats were randomly divided into four groups: normal group, model group (blank group), western medicine group (SASP group), and traditional Chinese medicine group (Qingchang Shuan group). There were 14 rats in each group. The rat ulcerative colitis model was established with TNBs 100 mg.kg-1 enema. On the third day, each group was given normal saline, sulfasalazine, and Qingchang suppository except for the normal group. The animals were sacrificed 7 days later. . Blood samples were taken from abdominal aorta to prepare serum. The levels of IL-1β in serum were determined by ELISA. Results: The serum IL-1β content in UC rats was significantly increased. Western medicine group and Chinese medicine group were able to reduce the content of IL-1β. Compared with the western medicine group, the Chinese medicine group P<0.01. Conclusion: The traditional Chinese medicine Qingchang suppository can reduce the serum IL-1β content and regulate the intestinal immunity, so as to alleviate the therapeutic purpose of UC.