极端低温对稻瘟菌的筛选及其生化机制初探

来源 :植物病理学报 | 被引量 : 0次 | 上传用户:liaodoctor
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
本研究利用来自我国南北稻区田间稻瘟菌菌株,对其进行了低温耐受性比较和统计分析。结果表明,南方与东北田间稻瘟菌在低温耐受性上存在明显差异:95%东北菌株可以忍受至少一周的-15℃低温处理,而相同条件下南方菌株仅有65%菌株存活。模拟东北冬季田间生境,选取10株低温敏感型菌株进行临界致死低温条件下的继代处理,发现-15℃条件下反复冻融处理55次后,其中8株低温耐受水平得到显著提高,且部分菌株的培养性状和抗药性相继发生改变。在此基础上,对抗性增强菌株的非原生质体蛋白进行双向电泳和差异蛋白质质谱分析,获得了响应低温的分泌蛋白及其指纹信息。本研究为进一步探索温度等环境因子对稻瘟菌的致变作用奠定了基础。 In this study, field blast strains from the north and south of our country were used to compare and analyze the low temperature tolerance. The results showed that there was a significant difference in low temperature tolerance between Magnaporthe grisea in the south and northeast China: 95% of northeastern strains could tolerate at least one week of low temperature treatment at -15 ℃, while only 65% ​​of strains of southern strains survived under the same conditions. In order to simulate the winter habitat in the northeastern China, 10 low temperature sensitive strains were selected for subculture at the critical lethal temperature. After repeated freezing and thawing treatment at -15 ℃ for 55 times, 8 low temperature tolerant plants were significantly increased Some strains of traits and drug resistance have changed. On this basis, the non-protoplast protein of the enhanced resistance strain was subjected to two-dimensional electrophoresis and differential protein mass spectrometry analysis, and the secretory protein and its fingerprint responding to low temperature were obtained. This study laid the foundation for further exploration of the mutagenic effects of environmental factors such as temperature on Magnaporthe grisea.
其他文献
目的 探讨17-β雌二醇治疗脊髓损伤的可能机制,以期为临床急性脊髓损伤的治疗提供理论依据.方法 建立原代培养的星形胶质细胞凋亡模型,将培养的细胞按随机排序的方法分为5组:
建宁县发展无患子规模化种植和加工符合我国能源多样化战略,是海峡西岸经济建设和建宁县发展县域经济的需要。分析了建宁县发展无患子产业面临的机遇和优势,同时针对存在的困
The purpose of this study is to investigate the function of a novel potassium transporter gene(NrHAK1) isolated from Nicotiana rustica roots using yeast complem
To understand genetic patterns of the morphological and physiological traits in flag leaf of barley, a double haploid (DH) population derived from the parents Y
Background: A major shortcoming in tissue engineered blood vessels (TEBVs) is the lack of healthy and easily attainable smooth muscle cells (SMCs). Smooth muscl
A method was proposed for the detection of outliers and influential observations in the framework of a mixed linear model, prior to the quantitative trait locus
产学研合作创新是企业、高校和科研院所按照“利益共享、风险共担、优势互补、共同发展”的原则而开展的技术创新活动.它是科技经济一体化的必然选择,也是国家创新体系的重要
2010年~2011年,在呼和浩特内蒙古农业大学牧草试验地,对根茎冰草新品系进行了2年的田间旱作播期试验,从2010年3月底至8月底,每隔1个月播种1次,共有6个播期处理.对物候期、生长
为了证明洗毛工艺是否对黏附于羊绒上口蹄疫病毒具有杀伤作用,本研究模拟山羊绒洗涤工艺,进行了不同的洗涤水温及作用时间、不同洗涤剂浓度及作用时间、不同的烘干温度及作用
乳腺浸润性小叶癌是一种较常见的乳腺癌类型,通常表现出显著的形态学、分子特征、以及独特的临床行为,其发生率也在逐年递增,近年来对小叶癌变异型的认识亦逐步加深.同时基于