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本研究利用来自我国南北稻区田间稻瘟菌菌株,对其进行了低温耐受性比较和统计分析。结果表明,南方与东北田间稻瘟菌在低温耐受性上存在明显差异:95%东北菌株可以忍受至少一周的-15℃低温处理,而相同条件下南方菌株仅有65%菌株存活。模拟东北冬季田间生境,选取10株低温敏感型菌株进行临界致死低温条件下的继代处理,发现-15℃条件下反复冻融处理55次后,其中8株低温耐受水平得到显著提高,且部分菌株的培养性状和抗药性相继发生改变。在此基础上,对抗性增强菌株的非原生质体蛋白进行双向电泳和差异蛋白质质谱分析,获得了响应低温的分泌蛋白及其指纹信息。本研究为进一步探索温度等环境因子对稻瘟菌的致变作用奠定了基础。
In this study, field blast strains from the north and south of our country were used to compare and analyze the low temperature tolerance. The results showed that there was a significant difference in low temperature tolerance between Magnaporthe grisea in the south and northeast China: 95% of northeastern strains could tolerate at least one week of low temperature treatment at -15 ℃, while only 65% of strains of southern strains survived under the same conditions. In order to simulate the winter habitat in the northeastern China, 10 low temperature sensitive strains were selected for subculture at the critical lethal temperature. After repeated freezing and thawing treatment at -15 ℃ for 55 times, 8 low temperature tolerant plants were significantly increased Some strains of traits and drug resistance have changed. On this basis, the non-protoplast protein of the enhanced resistance strain was subjected to two-dimensional electrophoresis and differential protein mass spectrometry analysis, and the secretory protein and its fingerprint responding to low temperature were obtained. This study laid the foundation for further exploration of the mutagenic effects of environmental factors such as temperature on Magnaporthe grisea.