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目的 :观察病毒感染对气道炎症、肺形态学的影响以及持续的气道反应性增高 ,探讨病毒感染与哮喘的关系。方法 :建立新生大鼠病毒感染模型 ,出生 5 d新生鼠超声雾化吸入柯萨奇病毒 B3(CVB3) ,接种后 10 d取血查 CVB3- Ig M;接种后 10d、30 d分别观察湿肺质量与鼠体质量比值 (L W/ BW)、气道肺病理学改变。 结果 :病毒组新生鼠血清中 CVB3- Ig M的 D值均在对照组 x+2 s以上接种后 10 d L W/ BW比值病毒组明显高于对照组 (P<0 .0 1)。病理学检查 :急性期炎症改变显著 ;接种后30 d仍有持续的形态学及细胞学改变。 结论 :新生鼠吸入 CVB3模型成立 ,并有持续的气道炎症及肺形态学改变。
OBJECTIVE: To observe the effects of virus infection on airway inflammation, lung morphology and persistent airway hyperresponsiveness to explore the relationship between viral infection and asthma. Methods: The newborn rat model of viral infection was established. Newborn mice were injected with Coxsackie virus B3 (CVB3) 5 days after birth and CVB3-Ig M 10 days after inoculation. The wet lungs were observed 10 and 30 days after inoculation Mass to body mass ratio (LW / BW), airway lung pathology changes. Results: The values of CVB3-Ig M in the serum of neonatal rats in the virus group were significantly higher than those in the control group (P <0.01) after 10 days of W / BW ratio in the control group. Pathological examination: Acute inflammation significantly changed; still 30 days after inoculation of morphological and cytological changes. Conclusion: CVB3 model was established in neonatal rats with inhaled airway inflammation and lung morphological changes.