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采用免疫细胞化学方法,对分离培养1、3、5、7d新生大鼠下丘脑促性腺激素释放激素(GnRH)免疫反应性神经元进行了研究。结果发现,在培养的1d即可检出GnRH神经元,GnRH神经元占培养中的神经元的12.1％～14.8％。GnRH神经元多为双极,有些突起末端可见生长锥。还发现GnRH神经元之间及其与其他神经元之间,存在有多种形式的接触。这些资料提示,培养中的GnRH神经元,保留了在体GnRH神经元的形态和功能特征,并为GnRH的脉冲式分泌及其调节提供了形态学依据。
Immunocytochemistry was used to study the hypothalamic gonadotropin-releasing hormone (GnRH) immunoreactive neurons isolated from neonatal rats at 1,3,5,7 days after culture. The results showed that GnRH neurons were detected on day 1 of culture, and GnRH neurons accounted for 12.1% -14.8% of cultured neurons. Most GnRH neurons are bipolar, some of the protruding end of the growth cone can be seen. It has also been found that there are many forms of contact between GnRH neurons and between them and other neurons. These data suggest that cultured GnRH neurons retain the morphological and functional characteristics of GnRH neurons in vivo and provide a morphological basis for the pulsatile secretion of GnRH and its regulation.