论文部分内容阅读
175KD葡萄糖磷脂酰肌醇(GPI)-联接糖蛋白CD109携带血小板系统的双等位基因Gov,目前检测Gov抗体依赖放射标记CD109的免疫沉淀技术。作者改良检测血小板抗原的单克隆抗体的血小板特异性固定技术,用以检测Gov抗体CD109表达ⅰ)在新鲜血小板上表达相对低,极少数为双倍表达ⅱ)PBS/EDTA保存的血小板减少,但血浆保存者增加;ⅲ)融化的冰冻保存血小板几无表达。检测605患者,其中305例新生儿同种免疫血小板减少(NAITP),60例输血后紫癜(PTP),和240例血小板输注无效(PR),发现14种Gov抗体(10 Gova抗体,4 Govb抗体)与本组中检测到的人类血小板(HPA)-5抗体一致。Gov抗体见于9例PR,两例PTP。目前认为PTP可能引起PR,CD109基因已被克隆,Gov抗原的分子已被测定,作者认为Gov同种抗体比以前报告的多,然而没有文献报道。CD109基因已被克隆,分子结构已测定。Gov同种抗体比以前想象的多。
175KD Glucose Phosphatidylinositol (GPI) - Gov, a dual allele linked to the glycoprotein CD109-carrying platelet system, currently detects immunoprecipitation of Gov antibodies on radiolabeled CD109. The authors modified a platelet-specific immobilization technique for monoclonal antibodies to platelet antigens to detect Gov antibody CD109 expression. I) Relatively low expression on fresh platelets, with a minority of double expression ii) Preserved thrombocytopenia in PBS / EDTA but Increased plasma reservoir; iii) Few expression of thawed platelets thawed. Sixty-five patients were tested, including 305 neonatal alloimmune thrombocytopenia (NAITP), 60 post-transfusion purpura (PTP), and 240 platelet transfusions (PR). 14 Gov antibodies (10 Gova antibody, 4 Govb Antibody) was consistent with the human platelet (HPA) -5 antibody detected in this group. Gov antibodies seen in 9 cases of PR, two cases of PTP. It is currently believed that PTP may cause PR, CD109 gene has been cloned, Gov antigen molecules have been determined, the authors believe that Gov alloantibody than previously reported, but no reported in the literature. The CD109 gene has been cloned and its molecular structure has been determined. Gov allo antibodies more than previously thought.