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以日本血吸虫尾蚴攻击感染C57BL/6小鼠,分别于感染后第1、2、3、4、5、7、9、11、13天收集小鼠脾组织,检测分析早期感染阶段小鼠脾中重要炎症因子的表达动态变化,观察脾不同亚群免疫细胞的变化趋势,分析先天免疫分子在血吸虫感染宿主免疫应答进程中的作用。RT-q PCR结果表明,GZMA、GZMB、GZMK等颗粒酶,CCL2、CXCL10、CXCL11等趋化因子,IL-12a、IL-4、IL-6、IL-10等白细胞介素在感染后均不同程度上调表达;流式细胞术结果表明,小鼠感染日本血吸虫后CD3~+CD4~+T细胞和CD3~+CD8~+T细胞比值一直高于未感染对照组;同时NK细胞亚群比例在感染后第5天和7天明显增多。这些免疫相关细胞和分子的变化可能在小鼠抗血吸虫感染中发挥了重要作用。本研究为探究日本血吸虫感染的先天免疫机制等积累了实验数据。
C57BL / 6 mice were challenged with Cercariae of Schistosoma japonicum and spleen tissues of mice were collected on the 1st, 2nd, 3rd, 4th, 5th, 7th, 9th, 11th, 11th day after infection respectively. The changes of immune cells in different subpopulations of spleen were observed. The function of innate immune molecules in the process of host immune response to schistosome infection was analyzed. RT-qPCR results showed that GZMA, GZMB, GZMK and other granzyme, CCL2, CXCL10, CXCL11 and other chemokines, IL-12a, IL-4, IL-6, IL-10 and other interleukin after infection are different The results of flow cytometry showed that the ratio of CD3 ~ + CD4 ~ + T cells and CD3 ~ + CD8 ~ + T cells in mice infected with Schistosoma japonicum was consistently higher than that in uninfected control group. The proportion of NK cell subpopulation On day 5 and 7 after infection, there was a significant increase. Changes in these immune-related cells and molecules may play an important role in the anti-schistosome infection in mice. This study accumulated experimental data to explore the innate immune mechanism of Schistosoma japonicum infection.