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目的 观察氨氯地平对人血管平滑肌细胞 (VSMC)丝裂素活化蛋白激酶 (MAPK)活性的影响。方法 取人乳腺下动脉培养VSMC ,用bFGF刺激细胞增殖 ,观察氨氯地平对bFGF激活的MAPK活性的影响 ,用 p42 / p44磷酸化抗体蛋白免疫印迹法测定MAPK活性。结果 bFGF对MAPK有显著激活作用 ,激活峰值出现在 5～ 15min ,维持至 3h ;此活化作用被MAPK激酶的特异抑制剂PD980 5 9抑制 ;无论瞬时或持久的bFGF激活的MAPK活性均被氨氯地平抑制。结论 氨氯地平通过MAPK信号转导途径抑制bFGF所致的细胞增殖
Objective To investigate the effect of amlodipine on the activity of mitogen - activated protein kinase (MAPK) in human vascular smooth muscle cells (VSMCs). Methods Human VSMCs were cultured with bFGF and the proliferation of VSMC was stimulated with bFGF. The effect of amlodipine on bFGF-activated MAPK activity was observed. The p42 / p44 phosphorylation antibody was used to measure the activity of MAPK. Results bFGF had a significant activation on MAPK. The activation peak appeared at 5-15min and maintained at 3h. This activation was inhibited by PD980-59, a specific inhibitor of MAPK kinase. Both transient and sustained bFGF-activated MAPK activity was inhibited by amniotic chloride Restrain the horizon. Conclusion Amlodipine inhibits the proliferation of cells induced by bFGF through the MAPK signal transduction pathway