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用霍乱毒素(CT)作为人类免疫佐剂的可能性取决于能否降低毒性而保留其佐剂性能,本研究旨在探索这一可能性。将仙台病毒与CT混合后用戊二醛处理(称一步法),透析、过琼脂糖6B凝胶柱除去游离的类毒素,收集OD280组分,超滤浓缩,即得到病毒-类毒素结合物。或将仙台病毒与CT分别用戊二醛处理、透析、混合,制备病毒与类毒素混合物。并以天然CT与戊二醛处理的病毒混合物作为对照,进行毒性、免疫佐剂性能及抗原性分析。采用一步法处理可使病毒与类毒素共价
The possibility of using cholera toxin (CT) as a human immunoadjuvant depends on whether toxicity can be reduced while preserving its adjuvant properties. This study aims to explore this possibility. The Sendai virus and CT mixed with glutaraldehyde treatment (referred to as one-step method), dialysis, Sepharose 6B gel column to remove free toxoid, collected OD280 components, concentrated by ultrafiltration to obtain a virus - toxoid conjugate . Or Sendai virus and CT were treated with glutaraldehyde, dialysis, mixing, preparation of virus and toxoid mixture. The virus mixture treated with native CT and glutaraldehyde was used as a control for toxicity, immunoadjuvant performance and antigenicity analysis. The one-step treatment allows the virus to be covalently attached to the toxoid