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目的探讨促血管生成素2(Ang-2)和Endoglin(Eng)在孕妇血清中的水平和在胎盘组织中的表达与胎儿生长受限(FGR)发病的关系。方法选取剖宫产分娩的FGR孕妇30例作为FGR组,同期剖宫产分娩的正常足月孕妇30例作为对照组。采用ELISA测定孕妇血清中Ang-2、可溶性Endoglin(sEng)的水平;采用SP检测胎盘组织中Ang-2、Eng的表达。结果 (1)FGR组血清Ang-2为(8.86±0.43)ng/ml,显著低于对照组的(19.31±0.25)ng/ml(P<0.01);FGR组sEng为(4.63±0.09)ng/ml,显著高于对照组的(2.77±0.09)ng/ml(P<0.01)。FGR组胎盘组织Ang-2的表达为118.17±1.59,显著低于对照组的151.64±1.64(P<0.01);FGR组胎盘组织Eng的表达为106.04±1.12,显著高于对照组的92.64±1.26(P<0.01)。(2)FGR组胎盘组织中Ang-2的表达与孕妇血清Ang-2水平呈正相关(r=0.93,P<0.01);FGR组胎盘组织中Eng的表达与孕妇血清中sEng水平呈正相关(r=0.88,P<0.01);FGR组胎盘组织中Ang-2的表达与Eng的表达无相关性;FGR组孕妇血清Ang-2水平与sEng水平无相关性。结论血清Ang-2水平和胎盘组织中表达显著降低,血清sEng和胎盘组织Eng表达水平显著升高可能参与了FGR的发病过程。
Objective To investigate the relationship between the levels of Ang-2 and Endoglin (Eng) in pregnant women serum and placenta tissues and the incidence of fetal growth restriction (FGR). Methods 30 pregnant women with FGR delivered by cesarean section were selected as FGR group and 30 normal pregnant women with cesarean delivery at the same period as control group. Serum levels of Ang-2 and soluble Endoglin (sEng) were measured by ELISA. The expression of Ang-2 and Eng in placenta was detected by SP method. Results The serum levels of Ang-2 in FGR group were (8.86 ± 0.43) ng / ml, which were significantly lower than those in control group (19.31 ± 0.25) ng / ml (4.63 ± 0.09) ng / / ml, which was significantly higher than that of the control group (2.77 ± 0.09) ng / ml (P <0.01). The expression of Ang-2 in placental tissue of FGR group was 118.17 ± 1.59, which was significantly lower than that of control group (151.64 ± 1.64) (P <0.01). The expression of Eng in placental tissue of FGR group was 106.04 ± 1.12, which was significantly higher than that of control group (92.64 ± 1.26) (P <0.01). (2) The expression of Ang-2 in placenta of FGR group was positively correlated with the level of serum Ang-2 in pregnant women (r = 0.93, P <0.01). The expression of Eng in placenta of FGR group was positively correlated with the level of sEng in pregnant women = 0.88, P <0.01). There was no correlation between the expression of Ang-2 and the expression of Ang in FGR group. There was no correlation between the level of Ang-2 and the level of sEng in FGR group. Conclusion Serum Ang-2 levels and placental tissue expression decreased significantly, serum Eng and placenta Eng expression levels may be involved in the pathogenesis of FGR.