The object of is to evaluate assimilable organic carbon(AOC) degradation rate by intensified biological technique in advanced water treatment. By artificially acclimating and cultivating strains attached onto carbon surface, the selected strains can be intensified for their degradation to organic matters. The research indicates that ozonation process increases AOC concentration considerably, however, it is beneficial to microdegradation. Temperature and empty bed contact time ( TEBC ) are two important factors affecting microbiology. From 14 to 27 ℃, intensified biological carbon can remove AOC better compared with granular activated carbon (GAC). Under identical TEBC, intensified technique increases more than 10% AOC reduction.