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采用CTAB法提取玉米及其制品的总DNA,用PCR方法检测其中的转基因成分如花椰菜花叶病毒(Cauliflowermosaicvirus,CaMV)35S启动子、根癌农杆菌(Agrobacteriumtumefaciens)胭脂碱合成酶基因(nos)终止子、根癌农杆菌CP4菌株的EPSPS基因、吸水链霉菌(Treptomyceshygroscopicus)bar基因及苏云金芽孢杆菌库尔斯塔克亚种(Bacillusthuringiensissubsp.kurstaki)cryIA(b)基因,筛选到阳性样品,并建立了几组玉米内源zein基因和转基因成分之间的多重PCR检测方法。结果表明,建立的多重PCR方法用于同时检测玉米内源基因和转基因成分是可行的,值得推广;虽然我国还未有已获准商品化生产的转基因玉米,但国外转基因玉米已流入福建省。
The total DNA of maize and its products was extracted by CTAB method, and the 35S promoter of Cauliflower mosaic virus (CaMV) 35S was detected by PCR. The termination of nos gene of Agrobacterium tumefaciens nopaline synthase , The EPSPS gene of Agrobacterium tumefaciens CP4 strain, the Treptomyceshygroscopicus bar gene and the Bacillus thuringiensis subsp. Kurstaki cryIA (b) gene were screened for positive samples and established Multiplex PCR Detection Between Several Zein Genes and Transgenic Compositions in Maize. The results showed that the established multiplex PCR method was feasible for the simultaneous detection of both maize endogenous genes and transgenic components, and was worth popularizing. Although there are no genetically modified maize that have been approved for commercial production in China, foreign genetically modified maize has been introduced into Fujian Province.