论文部分内容阅读
本文评价了一种培养氯消毒污水中类大肠菌群的新方法,即A-1培养基一步培养法。(译者注:A-1培养基的成份为:乳糖5.0 g,胰蛋白胨20.0 g,氯化钠5.0 g,水杨苷0.5 g,TritonX-100 1.0 ml,蒸馏水1,000 ml)将水样接种到A-1培养基后,35℃下预培养3小时,再转至44.5℃培养21小时即可得出结果。从威斯康星州污水处理厂采集了13个样本,用A-1培养基一步培养法和两步MPN法对比测定样本中的粪大肠菌群,每个样本各作5份重复试验。配对t检验分析结果表明:两法
In this paper, a new method of culturing coliform bacteria in chlorinated wastewater was evaluated, that is, A-1 medium one-step culture method. Water samples were inoculated into the medium of A-1 medium: 5.0 g of lactose, 20.0 g of tryptone, 5.0 g of sodium chloride, 0.5 g of salicin, 1.0 ml of Triton X-100 and 1,000 ml of distilled water A-1 medium, pre-incubated at 35 ° C for 3 hours, and then transferred to 44.5 ° C for 21 hours to obtain the result. Thirteen samples were collected from the Wisconsin Sewage Treatment Plant. Fecal coliforms were determined by one-step A-1 culture and two-step MPN. Five replicates were tested for each sample. Paired t test results show that: two methods