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分别用过氧化氢和溶菌酶降解壳聚糖,制备得到了系列分子量(3.8×105,7.8×104,4.8×104,1.7×104,2.3×103)的壳聚糖样品.采用平板法研究了壳聚糖系列样品对大叶黄杨炭疽病、沿阶草炭疽病、广玉兰炭疽病和对节白蜡腐烂病等庭院植物病原真菌的体外抗菌性能.结果表明:水不溶性壳聚糖酸溶液均表现出抑菌活性,且随着浓度的增大而抑菌活性增强;中等分子量(7.8×104)壳聚糖比大分子量(3.8×105)壳聚糖和低分子量(1.7×104,2.3×103)壳聚糖抗菌作用强.
A series of chitosan samples with molecular weights (3.8 × 105, 7.8 × 104, 4.8 × 104, 1.7 × 104, 2.3 × 103) were prepared by hydrogen peroxide and lysozyme degradation, respectively. Chitosan In vitro antibacterial activity of chitosan samples against garden plant pathogenic fungi such as Anthracnophus euchroma, Stephanic grass anthracnose, Magnolia grandiflora and Alternaria alternata were studied in vitro.The results showed that water insoluble chitosan acid solution The antibacterial activity of the medium molecular weight chitosan (7.8 × 104) was higher than that of the high molecular weight chitosan (3.8 × 105) and the low molecular weight (1.7 × 104, 2.3 × 103) Chitosan antibacterial effect.