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目的 检测以恶性疟原虫裂殖子表面蛋白 1的 17区片段基因为基础的复合核酸疫苗(分泌性的VR10 12 /TPA/HG MSP1 17和非分泌性的VR10 12 /HG MSP1 17)诱导小鼠的体液免疫反应和免疫血清对疟原虫生长的抑制能力。方法 以 2 0 0 μg/10 0 μl或 10 0 μg/10 0 μl每次每只VR10 12 /HG MSP1 17或VR10 12 /TPA/HG MSP1 17肌注免疫BALB/c或C5 7BL/6小鼠。用ELISA间接法测定小鼠血清的特异性抗体 ,用体外抑制试验检测免疫血清抑制疟原虫生长效果。结果 经 3次 10 0 μg/10 0 μl每次每只VR10 12 /HG MSP1 17免疫后 ,BALB/c小鼠和C5 7BL/6小鼠均产生了明显的HG和YMSP119抗体。但总体抗体水平不高。BALB/c小鼠经 3次 2 0 0 μg/10 0 μl每次每只的VR10 12 /HG MSP1 17免疫后 ,产生了较高的HG抗体 ,但MSP1 17的抗体无明显变化 ,经 3次 2 0 0 μg/10 0 μl每次每只VR10 12 /TPA/HG MSP1 17免疫后 ,仅产生较低的HG抗体 ,无MSP1 17抗体的产生。用 2 0 0 μg/10 0 μlVR10 12 /HG MSP1 17免疫的BALB/c小鼠血清做体外抑制试验 ,结果抑制效果明显。结论 VR10 12 /HG MSP1 17比VR10 12 /TPA/HG MSP 17具有更强的免疫原性 ,其免疫鼠血清能明显地抑制疟原虫生长
Objective To examine the effect of recombinant nucleic acid vaccine (secretory VR10 12 / TPA / HG MSP1 17 and nonsecretive VR10 12 / HG MSP1 17) based on the gene encoding region 17 of Plasmodium falciparum merozoite surface protein 1 Humoral immune response and immune serum inhibition of Plasmodium growth. Methods BALB / c or C5 7BL / 6 mice were immunized intraperitoneally with 200 μg / 100 μl or 100 μg / 100 μl of each VR10 12 / HG MSP1 17 or VR10 12 / TPA / HG MSP1 17 . The specific antibodies of mouse serum were detected by ELISA indirect method, and the inhibition effect of immune serum on Plasmodium growth was tested by in vitro inhibition test. Results Both BALB / c mice and C5 7BL / 6 mice developed significant HG and YMSP119 antibodies after three immunizations with 10 0 μg / 100 μl of each VR10 12 / HG MSP1 17. However, the overall antibody level is not high. BALB / c mice were immunized with 3 times of 200 μg / 100 μl of VR10 12 / HG MSP1 17 each time, resulting in a higher HG antibody but no significant change in the antibody of MSP1 17. After 3 times After immunization with 200 μg / 100 μl of each VR10 12 / TPA / HG MSP1 17, only lower HG antibodies were produced, with no production of MSP1 17 antibodies. BALB / c mouse serum immunized with 200 μg / 10 μL of VR10 12 / HG MSP1 17 was used for in vitro inhibition test, and the results showed obvious inhibitory effect. Conclusion VR10 12 / HG MSP1 17 is more immunogenic than VR10 12 / TPA / HG MSP 17, and its immunized rat serum can significantly inhibit the growth of malaria parasite