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目的建立同时检测连翘、栀子主要有效成分的HPLC方法,考察药对连翘与栀子配伍前后主要化学成分的变化情况。方法采用高效液相色谱法对连翘、栀子的单味药提取液及连翘、栀子药对提取液中连翘苷和栀子苷的含量进行了测定。结果各成分提取量在单药煎液与药对煎液中变化不明显,药对提取液中栀子苷和连翘苷含量与单味药提取液比较,栀子苷在96.89%到98.24%之间,连翘苷在96.34%到102.67%之间(n=3)。结论建立了同时检测连翘、栀子主要有效成分的HPLC方法,为评价煎液中连翘苷和栀子苷质量提供依据,为进一步体内试验提供数据参考。
OBJECTIVE To establish a HPLC method for simultaneous determination of main active ingredients of Forsythia suspensa and Gardenia jasminoides, and to study the changes of main chemical components before and after compatibility of Forsythia suspensa and Fructus Gardeniae. Methods The contents of forsythin and geniposide in Forsythia suspensa and Fructus Gardeniae extract, Forsythia suspense and Fructus Gardeniae extract were determined by HPLC. Results The amount of each component in decoction of single decoction and medicine on Decoction did not change significantly, the drug extract of Geniposide and forsythin content and single extract comparison, geniposide at 96.89% to 98.24% Forsythiaside was between 96.34% and 102.67% (n = 3). Conclusion HPLC method was established to detect the main active ingredients of Forsythia suspense and Gardenia jasminoides in order to provide a basis for evaluating the quality of Forsythin and Geniposide in decoction and provide reference for further in vivo experiments.