本文介绍了一种用来诊断早孕的特异和高度敏感的放射免疫测定方法,该方法是通过测定人的血浆、尿、羊水和乳汁中的特异的β_1-糖蛋白(简称SP1) 。SP1是由胎盘滋养层的合体细胞合成,分子量大约为90,000,其中含糖量29. 3%采用由~(125) Ⅰ标记的高度纯化的 SP1作为抗原和相应的抗血清。标准系由晚孕血清稀释于马血清中组成。进行放射免疫测定时的最后保温量为500 This article describes a specific and highly sensitive radioimmunoassay method used to diagnose early pregnancy by measuring specific β_1-glycoprotein (SP1) in human plasma, urine, amniotic fluid and milk. SP1 was synthesized by a syncytiotrophoblast of the placenta with a molecular weight of about 90,000 with a sugar content of 29.3% using highly purified SP1 labeled with 125I as the antigen and the corresponding antisera. The standard is made up of serum diluted in horse serum in late pregnancy. The final amount of insulation for radioimmunoassay was 500