In order to further investigate the effect of natural antioxidant tea polyphenols （TP） on cardiovascular and nervous systems, the effects of TP on cardiac function of isolated working heart in rat, calcium inward current in cultured rat sympathetic neuron and energy metabolism in mouse brain were observed.

①In isolated working heart, the changes of cardiac function before and after adding TP （1mg/L） to the perfusate were compared; ②Effect of TP on cellular calcium inward current was recorded using whole-cell patch-clamp technique;　③Effect of TP on energy metabolism in mouse brain was studied using ³¹P-nuclear magnetic resonance （NMR） technique.

① Aortic flow （AF）， left ventricular systolic pressure （LVSP） and the maximal rate of rise in LVSP （+dp/dt_max） of the rat heart were significantly increased after administration of TP; ②The calcium inward current was remarkbly enhanced by TP; ③TP could modulate energy metabolism in mouse brain, especially increase the phosphocreatine concentration.

This study further confirms the cardiac inotropic action of TP, which might be related to the activating effect on calcium channel. In addition, TP is helpful in energy metabolism of brain.