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目的探讨14-3-3蛋白各亚型在人胰腺导管腺癌组织中的表达情况及其临床意义。方法采用石蜡包埋组织进行免疫组化染色,分析14-3-3蛋白各亚型在30例胰腺癌和19例胰腺癌旁正常组织中的表达状态,探讨其与胰腺癌临床病理参数之间的关系。结果 14-3-3蛋白β、γ、ε、ζ、η、θ和σ7个亚型在胰腺癌组织原发灶中表达阳性率分别为70%(21/30)、83.33%(25/30)、26.67%(8/30)、73.33%(22/30)、33.33%(10/30)、30%(9/30)和43.33%(13/30)。其中癌旁正常胰腺组织的β、γ、ζ、η和σ亚型阳性率与胰腺癌组织比较差异显著(P<0.05),ε和θ亚型阳性率比较差异不显著(P>0.05);14-3-3蛋白阳性表达率与胰腺癌的分化程度、恶性级别及淋巴结转移均无明显关系(P>0.05)。结论 14-3-3蛋白7个亚型中有5个在胰腺癌组织中高表达,表明该蛋白在胰腺癌的发生过程中具有重要作用,并且有可能成为胰腺癌基因治疗新的靶点。
Objective To investigate the expression and clinical significance of 14-3-3 protein subtypes in human pancreatic ductal adenocarcinoma. Methods Immunohistochemical staining of paraffin-embedded tissues was performed to analyze the expression status of 14-3-3 protein subtypes in 30 cases of pancreatic cancer and 19 cases of normal tissues adjacent to pancreatic cancer, and explore the relationship between the 14-3-3 protein subtypes and clinicopathological parameters of pancreatic cancer. Relationship. Results The positive rates of 14-3-3 protein β, γ, ε, ζ, η, θ and σ 7 subtypes in primary pancreatic cancer tissues were 70% (21/30) and 83.33% (25/30) respectively. ), 26.67% (8/30), 73.33% (22/30), 33.33% (10/30), 30% (9/30) and 43.33% (13/30). The positive rates of β, γ, ζ, η and σ subtypes in normal pancreatic tissues were significantly different from those in pancreatic cancer tissues (P<0.05). The positive rates of ε and θ subtypes were not significantly different (P>0.05). The positive expression rate of 14-3-3 protein was not significantly related to the degree of differentiation, malignant grade and lymph node metastasis of pancreatic cancer (P>0.05). Conclusion Five out of seven subtypes of 14-3-3 protein are highly expressed in pancreatic cancer, indicating that this protein plays an important role in the development of pancreatic cancer and may become a new target for gene therapy of pancreatic cancer.