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分别采用5 mW.mm-2He-Ne激光辐照1、0.08 kJ.m-2.d-1增强紫外线-B(UV-B)辐射及二者组合对“晋麦8号”小麦幼苗进行处理,5 d后测定各处理幼苗叶片和根的RNA酶(RNase)和DNA酶(DNase)活性变化。结果表明,经UV-B辐射后,小麦幼苗叶片和根的核酸酶活性均有明显升高,电泳图谱中其酶谱带均没有增加,只是酶活性的升高;复合处理的小麦幼苗其酶活性较之单独UV-B辐射的有显著或极显著降低,电泳后的酶谱带也没有减少,只是活性下降。因此认为一定剂量的He-Ne激光辐照能抑制UV-B辐射后小麦幼苗核酸酶活性的升高。
Radiation of UV-B (UV-B) at a dose of 1.0.08 kJ.m-2.d-1 and UV-B radiation of 5 mW.mm-2He-Ne were performed on wheat seedlings of “Jinmai No.8” After 5 days, the changes of RNase and DNase activities in the leaves and roots of each seedling were determined. The results showed that after UV-B radiation, the nuclease activity of wheat seedling leaves and roots were significantly increased, the electrophoretic spectrum of the enzyme bands did not increase, but increased enzyme activity; composite treatment of wheat seedlings enzyme Compared with the UV-B radiation alone, there was a significant or a significant decrease in the activity of the enzyme band after electrophoresis did not reduce, but the activity decreased. Therefore, a certain dose of He-Ne laser irradiation can inhibit the increase of nuclease activity of wheat seedlings after UV-B radiation.