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目的:建立同时测定复方儿茶酊中儿茶素与原儿茶素含量的方法。方法:采用高效液相色谱法。色谱柱为YMC ODS(250mm×4.6mm,5μm),流动相为0.04mol·L-1枸橼酸溶液-N,N-二甲基甲酰胺-四氢呋喃(45∶8∶2,V/V/V),流速为1.0mL·min-1,检测波长为280nm,柱温为35℃。结果:儿茶素与原儿茶素的检测浓度分别在0.0518～0.2590、0.0101～0.2030mg·mL-1(r均为0.9999)范围内与各自峰面积积分值呈良好的线性关系;平均加样回收率分别为101.63%和100.49%,RSD分别为1.27%和1.12%(n均为9)。结论:本方法简便、准确、稳定,能有效控制复方儿茶酊的质量。
Objective: To establish a method for the simultaneous determination of catechin and protocatechin in compound catechu tincture. Methods: Using high performance liquid chromatography. The chromatographic column was YMC ODS (250 mm × 4.6 mm, 5 μm) with a mobile phase of 0.04 mol·L -1 citric acid solution of N, N-dimethylformamide-tetrahydrofuran (45: 8: V). The flow rate was 1.0 mL · min-1, the detection wavelength was 280 nm and the column temperature was 35 ℃. Results: The concentrations of catechin and protocatechin were linearly correlated with their respective peak areas in the range of 0.0518-0.2590,0.0101-0.2030 mg · mL-1 (r = 0.9999) The recoveries were 101.63% and 100.49%, respectively, with RSDs of 1.27% and 1.12%, respectively (n = 9). Conclusion: The method is simple, accurate and stable, and can effectively control the quality of compound catechu tincture.