论文部分内容阅读
目的:建立檀香挥发油的指纹图谱分析方法,为檀香药材的质量评价提供方法与依据。方法:采用乙醚冷浸法提取檀香挥发油,应用GC-MS联用技术,采用HP-5弹性石英毛细管柱(30 m×0.32 mm,0.25μm),程序升温(柱温90℃,以5℃·min~(-1)升至160℃,再以0.5℃·min~(-1)升至165℃,然后再以1℃·min~(-1)升至180℃,最后10℃·min~(-1)升至230℃),建立挥发油的共有峰指纹图谱,采用中南大学指纹图谱相似度评价软件和SPSS 20.0软件对不同来源的檀香油对照品及14批样品挥发油分别进行相似度评价和聚类分析。结果:所建立的檀香挥发油GC-MS指纹图谱有较好的重复性、精密度和稳定性(RSD均小于5%)。檀香油对照品及14批样品挥发油中α-檀香醇、β-檀香醇相对百分含量分别为34.225%~51.156%、20.897%~26.857%。以檀香油对照品为参照,14批样品挥发油相关系数为0.622~0.984,相合系数为0.628~0.984,但同一基地采集的5批檀香样品挥发油相关系数为0.924~0.984,相合系数为0.934~0.984,市购檀香样品挥发油相关系数为0.622~0.868,相合系数为0.628~0.838。通过聚类分析,在类间距离=15时,檀香油对照品及14批样品可以分为两类,檀香油对照品及S2~S9号样品为一类,S10~S15号样品为一类。结论:所建立的檀香挥发油GC-MS指纹图谱法为中药檀香的质量控制提供了一种有效手段。同一种植基地檀香样品的指纹图谱相似度较高,质量较均一;市售檀香样品的指纹图谱相似度较低,质量参差不齐。
Objective: To establish the fingerprinting analysis method of essential oil of sandalwood, provide the method and basis for the quality evaluation of sandalwood medicine. Methods: Volatile oil of sandalwood was extracted by cold soaking with ethyl ether. GC-MS coupled with HP-5 elastic quartz capillary column (30 m × 0.32 mm, 0.25 μm) · Min -1 increased to 160 ℃ and then increased to 165 ℃ at 0.5 ℃ · min -1 and then increased to 180 ℃ at 1 ℃ · min -1 ~ (-1) to 230 ℃). The fingerprint of volatile oil was established. The fingerprint similarity evaluation software of Central South University and SPSS 20.0 software were used to evaluate the similarity of sandalwood oil from different sources and 14 batches of volatile oil And cluster analysis. Results: GC-MS fingerprints of the essential oil of sandalwood had good repeatability, precision and stability (RSD less than 5%). The relative percentage of α-santalol and β-santalol in the control oil of sandalwood oil and the volatile oil of 14 batches were 34.225% -51.156% and 20.897% -26.857%, respectively. The correlation coefficients of volatile oil of 14 batches of samples were 0.622-0.984 with the correlation coefficients of 0.628-0.984. However, the correlation coefficients of volatile oil of five batches of sandalwood collected from the same base were 0.924-0.984 and the coincidence coefficients were 0.934-0.984 , The correlation coefficients of volatile oil of commercially available sandalwood samples were 0.622-0.868, and the consistency coefficients were 0.628-0.838. According to the cluster analysis, sandalwood oil reference and 14 batches of samples can be divided into two categories when the distance between classes = 15, sandalwood oil reference and S2 ~ S9 samples, and S10 ~ S15 samples. Conclusion: The established GC-MS fingerprint of essential oil of sandalwood provides an effective method for the quality control of traditional Chinese medicine sandalwood. The fingerprints of sandalwood samples from the same planting site had higher similarity and quality, and the fingerprints of commercially available sandalwood samples had lower similarity and uneven quality.