目的:探讨中电导钙激动钾离子通道(SK4)在原发性肝细胞癌(HCC)中的表达情况及意义。方法:收集46例HCC患者手术标本的HCC组织与癌旁组织。用免疫组化法检测两种组织中SK4与血管内皮细胞生长因子(VEGF)在的表达,分析HCC组织中SK4与VEGF表达的相关性;用realtime PCR法检测两种组织中SK4 mRNA的表达,并分析SK4 mRNA表达与HCC临床病理因素的关系。用Western blot法检测两种组织中SK4蛋白的表达。结果:免疫组化结果显示,肝癌组织中SK4和VEGF的阳性表达率均明显高于癌旁组织(均P<0.05),且在HCC组织中SK4和VEGF阳性表达呈正相关(r=0.364,P<0.05);real-time PCR结果显示,HCC组织中SK4 mRNA表达水平较癌旁组织明显上调(P<0.05),且SK4 mRNA的高表达与肿瘤低分化及门静脉癌栓有关(均P<0.05);Western blot结果显示,SK4蛋白在HCC细胞膜的表达明显高于癌旁肝细胞,但两者胞质SK4水平无明显差异。结论:SK4在HCC组织中表达增高,其可能通过上调VEGF的表达等途径促进HCC细胞的侵袭转移。 Objective: To investigate the expression of calcium-sensitive potassium channel (SK4) in primary hepatocellular carcinoma (HCC) and its significance. Methods: HCC tissues and adjacent tissues from 46 HCC patients were collected. Immunohistochemistry was used to detect the expression of SK4 and vascular endothelial growth factor (VEGF) in the two tissues. The correlation between the expression of SK4 and VEGF in HCC tissues was analyzed. The expression of SK4 mRNA in both tissues was detected by realtime PCR. And analyze the relationship between SK4 mRNA expression and clinicopathological factors of HCC. Western blot was used to detect the expression of SK4 protein in both tissues. Results: Immunohistochemical results showed that the positive rates of SK4 and VEGF in HCC tissues were significantly higher than those in paracancer tissues (all P <0.05), and the positive expressions of SK4 and VEGF in HCC tissues were positively correlated (r = 0.364, P <0.05). The results of real-time PCR showed that the expression of SK4 mRNA in HCC tissues was significantly up-regulated compared with that in adjacent non-cancerous tissues (P <0.05), and the high expression of SK4 mRNA was associated with poorly differentiated tumor and portal vein tumor thrombus ). The results of Western blot showed that the expression of SK4 protein in HCC cell membrane was significantly higher than that in paracancerous hepatocytes, but there was no significant difference between the two cytosolic SK4 levels. Conclusion: The expression of SK4 in HCC tissues is increased, which may promote the invasion and metastasis of HCC cells by up-regulating the expression of VEGF.