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应用口腔颌面部恶性肿瘤ＣＴＸ免疫抑制鼠ＳＲＣ移植瘤，经ＰＭ、ＰＶＦ、ＣＶＰ治疗后结合癌细胞形态的改变、Ａｇ—ＮＯＲ及计算机自动图象分析仪测定判断药物的敏感性。结果显示：①形态学观察治疗后癌细胞呈明显退行性改变，核肿胀，核边界不清，核染色质颗粒模糊，核有固缩。未化疗对照组，瘤细胞生长活跃核分裂相多见，排列密集。②Ａｇ—ＮＯＲ平均计数与细胞增殖活性，核仁融合功能，细胞核倍体数等有关。化疗敏感组Ａｇ—ＮＯＲ平均计数明显减少。③Ｆｅｕｌｇｅｎ染色图象分析检测，化疗敏感组ＤＮＡ指数明显偏低。提示联合化疗敏感组严重抑制细胞ＤＮＡ合成。
The application of CTX immunosuppression in mice with oral and maxillofacial malignancies for SRC transplanted tumors was determined by combination of PM, PVF, and CVP with changes in the morphology of cancer cells, and Ag-NOR and computerized automatic image analyzers were used to determine drug sensitivity. The results showed that: 1 After morphological observation, the cancer cells showed marked degenerative changes, nuclear swelling, unclear nuclear borders, blurred nuclear chromatin particles, and nuclear condensation. In the non-chemotherapy control group, the active mitotic cells of the tumor cells were more common and densely arranged. The average count of 2Ag-NOR was related to cell proliferation activity, nucleolar fusion function, and the number of nuclear ploidy. The average count of Ag-NOR in the chemotherapy-sensitive group was significantly reduced. 3Feulgen staining image analysis showed that the DNA index of chemotherapy-sensitive group was significantly lower. It is suggested that the combination chemotherapy sensitive group severely inhibits cell DNA synthesis.