目的:应用全基因组测序（WGS）和脉冲场凝胶电泳（PFGE）分型技术鉴别新疆一起疑似聚集性伤寒疫情。方法:通过新疆维吾尔自治区传染病报告系统收集5例伤寒病例，对5例病例的流行病学史进行回顾性调查，重点针对病例的诊断过程以及疑似感染来源进行调查，同时对5株分离到的伤寒沙门菌进行 WGS和PFGE分析和聚类，最后结合流行病学调查结果，判定疫情性质。结果:5株伤寒沙门菌的基因组序列、PFGE图谱、耐药表型和耐药基因高度一致；流行病学调查发现，5例病例曾于同一天在同一家餐馆就餐。结论:结合伤寒最长潜伏期、病原菌分子分型和流行病学调查结果，可初步判定此次疫情为一起通过就餐食物而感染伤寒沙门菌的聚集性疫情。“,”Objective:To identify a suspected clustered Typhoid fever by whole genome sequencing(WGS) and pulsed field gel electrophoresis (PFGE) subtyping.Methods:The nature of the epidemic was determined by combination of subtyping results of isolates and epidemiological information.Results:Five n S. typhimurium isolates showed identical PFGE patterns and almost the same whole genome sequence. Epidemiological survey showed that five cases had dined in the same restaurant on the same day.n Conclusion:Combined with the longest incubation period of typhoid fever, molecular subtyping of pathogenic bacteria and the field epidemiological survey, it can be preliminarily determined that the five cases have common infection sources.