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目的探讨邻苯二甲酸二(2-乙基己)酯(di-2-ethylhexyl phthalate,DEHP)对青春前期雄性大鼠的生殖毒性。方法将32只健康21日龄清洁级雄性SD大鼠按体重随机分为4组,分别为溶剂对照(玉米油)组和250、500、1 000 mg/kg DEHP染毒组,每组8只。采用灌胃方式进行染毒,染毒容量为10 ml/kg,每天1次,连续染毒4周。采用CASA精子分析系统分析精子活力相关指标。结果与对照组比较,1 000 mg/kg DEHP染毒组大鼠的精子密度、精子活动率、活动精子密度、最大侧摆幅度、直线运动的精子密度及A级精子百分率均较低,而D级精子百分率较高;250 mg/kg DEHP染毒组平均直线运动速度、精子运动的向前性均较低;500、1 000 mg/kg DEHP染毒组直线运动的精子个数均较低,差异均有统计学意义(P<0.05);而各剂量DEHP染毒组B、C级精子比例和平均曲线运动速度、平均路径速度、平均侧摆幅度、平均鞭打频率、运动的直线性、运动的摆动性、平均移动角度、直线运动精子活率均无明显改变。结论 DEHP对青春前期雄性大鼠精子活力产生明显的毒性作用,从而影响雄性的生殖功能。
Objective To investigate the reproductive toxicity of di-2-ethylhexyl phthalate (DEHP) to pre-adolescent male rats. Methods Thirty-two healthy 21-day-old male Sprague-Dawley rats were randomly divided into four groups according to body weight: solvent control (corn oil) and 250, 500 and 1 000 mg / kg DEHP exposure groups, . Gavage by way of exposure, exposure capacity of 10 ml / kg, 1 day, continuous exposure to 4 weeks. CASA sperm analysis system analysis of sperm motility related indicators. Results Compared with the control group, the sperm density, sperm motility, motile sperm density, maximum lateral swing amplitude, linear sperm motility and percentage of grade A sperm were all lower in 1 000 mg / kg DEHP-treated rats, while D The percentage of sperm was higher; the average linear velocity of 250 mg / kg DEHP exposure group was lower than that of sperm motility; the number of spermatozoa in straight line of 500 and 1 000 mg / kg DEHP exposure groups was lower, (P <0.05). However, the ratios of B and C spermatozoa and average curve velocity, mean path velocity, mean lateral swing amplitude, average whipping frequency, linear motion of exercise, Of the swinging, the average movement angle, linear motility sperm motility no significant change. Conclusion DEHP has a significant toxic effect on the sperm motility of pre-adolescent male rats and thus affects male reproductive function.