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目的:观察大豆异黄酮对脑缺血再灌注大鼠海马神经再生的影响。方法:线栓法建立成年大鼠脑缺血再灌注模型,分别给予大豆异黄酮(SI)或联合给予他莫昔芬(tamoxifen,TX)和SI,BrdU免疫荧光法标记新生海马神经细胞,免疫组化法和western blot检测海马区phosphorylated CREB(pCREB)的表达。结果:大豆异黄酮(SI)组和联合给药(SI+TX)组大鼠海马区BrdU+细胞数显著增加(与模型组比,P<0.01),且SI组大鼠海马区BrdU+细胞数明显多于SI+TX组(P<0.05)。SI组大鼠海马区BrdU+/pCREB+的细胞数量明显增多,pCREB的表达也明显增加(与模型组比较,P<0.05);SI+TX组大鼠海马区BrdU+/pCREB+的细胞数量明显减少(与大豆异黄酮组比较,P<0.05),pCREB的表达明显减少(与模型组比,P<0.05)。结论:长期给予大豆异黄酮可以使脑缺血再灌注海马神经再生,这种作用可能与其雌激素活性、促进CREB的磷酸化以维持神经再生、存活或修复的信号有关。
Objective: To observe the effects of soy isoflavones on hippocampal neurogenesis after cerebral ischemia-reperfusion in rats. Methods: The model of cerebral ischemia-reperfusion in adult rats was established by thread occlusion. Neonatal rat hippocampal neurons were immunostained with either soy isoflavone (SI) or tamoxifen (TX) and SI, BrdU respectively The expression of phosphorylated CREB (pCREB) in hippocampus was detected by histochemistry and western blot. Results: The number of BrdU + cells in hippocampus of SI group and SI + TX group increased significantly (compared with model group, P <0.01), and the number of BrdU + cells in hippocampus of SI group was significantly More than SI + TX group (P <0.05). The number of BrdU + / pCREB + cells in hippocampus of SI group was significantly increased and the expression of pCREB was significantly increased (P <0.05 compared with model group). The number of BrdU + / pCREB + cells in hippocampus of SI + TX group was significantly decreased Soy isoflavones group, P <0.05), pCREB expression was significantly reduced (compared with the model group, P <0.05). CONCLUSION: Long-term administration of soy isoflavones can regenerate hippocampal neurons after cerebral ischemia and reperfusion. This effect may be related to its estrogen activity, phosphorylation of CREB to maintain the signal of nerve regeneration, survival or repair.