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目的探讨Th17/Treg细胞在儿童支原体肺炎发病机制中的作用。方法流式细胞术检测35例急性期、30例恢复期的支原体肺炎患儿和20例同期健康体检儿童外周静脉血Th17/Treg细胞频数,采用ELISA双抗体夹心法检测血清中IL-17、IL-10和TGF-β细胞因子的表达水平。结果 MPP患儿急性期组外周血的Th17细胞百分率均明显高于恢复期组和对照组;而Treg细胞的百分率明显低于恢复期组和对照组;MPP患儿急性期组外周血的IL-17A表达均明显高于恢复期组和对照组;而TGF-β和IL-10表达明显低于恢复期组和对照组,上述差异均有统计学意义(P<0.01)。MPP急性期组和恢复期组的外周血Th17细胞百分率和血清细胞因子IL-17的表达水平呈正相关(r=0.724,r=0.738,P<0.01);Treg细胞百分率与TGF-β表达水平呈正相关(r=0.826,r=0.814,P<0.01)。结论 Th17/Treg细胞及其细胞因子失衡可能是儿童支原体肺炎发病的重要原因。
Objective To investigate the role of Th17 / Treg cells in the pathogenesis of children with Mycoplasma pneumoniae. Methods The frequency of Th17 / Treg cells in 35 patients with acute myeloid leukemia and 30 patients with convalescent mycoplasma pneumonia and 20 healthy children were detected by flow cytometry. The levels of IL-17 and IL-17 -10 and TGF-β cytokine expression levels. Results The percentages of Th17 cells in the peripheral blood of MPP children were significantly higher than those in the recovery group and the control group. The percentage of Treg cells was significantly lower in the MPP children than in the recovery group and the control group. The levels of IL- 17A expression were significantly higher than the recovery group and the control group; while TGF-β and IL-10 expression was significantly lower than the recovery group and the control group, the difference was statistically significant (P <0.01). The percentage of Th17 cells in peripheral blood and the level of serum cytokine IL-17 in MPP acute phase group and convalescent phase group were positively correlated (r = 0.724, r = 0.738, P <0.01). The percentage of Treg cells and TGF- (R = 0.826, r = 0.814, P <0.01). Conclusion The imbalance of Th17 / Treg cells and cytokines may be an important cause of mycoplasma pneumonia in children.