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目的:探讨复方儿茶止泻霜对腹泻大鼠结肠AQP1表达的影响。方法:HE染色观察比较各组大鼠结肠黏膜组织形态学变化,采用免疫组化法比较腹泻模型组(DM)、正常对照组(NC)及低(L)、高(H)剂量药物治疗组、阳性对照药物治疗组(PC)AQP1的表达。结果:形态学观察中NC组大鼠近端结肠黏膜形态规整,单层柱状上皮排列整齐;DM组中黏膜上皮明显水肿,间质明显水肿、充血、渗出、大量淋巴细胞等炎性细胞浸润;柱状上皮细胞之间界限不清、部分上皮坏死、脱落;H组大鼠结肠受损结构明显恢复,大部分结肠内仅见极少量渗出及淋巴细胞浸润,柱状上皮细胞排列整齐。免疫组化法提示:DM组AQP1表达低于L组、PC组和H组,L组AQP1表达低于H组和PC(P<0.01),但H组和PC与NC组间无明显差异(P>0.05)。结论:复方儿茶止泻霜上调腹泻大鼠结肠AQP1的表达。
Objective: To investigate the effect of compound catechu diarrhea cream on the expression of AQP1 in colon of diarrhea rats. Methods: The morphological changes of colonic mucosa of rats in each group were observed by HE staining. The pathological changes of colonic mucosa were observed by immunohistochemical method in DM, NC, L and H groups , Positive control drug treatment group (PC) AQP1 expression. Results: Morphological observation showed that the morphology of proximal colonic mucosa of NC rats was regular and the monolayer columnar epithelium was arranged neatly. In DM group, there was obvious edema in mucosal epithelium, obvious edema in interstitium, hyperemia, exudation and inflammatory cell infiltration ; Columnar epithelial cells between the unclear boundaries, part of the epithelial necrosis, shedding; colon injury group H rats significantly recovered, most of the colon only a very small amount of exudate and lymphocyte infiltration, columnar epithelial cells arranged in neat rows. Immunohistochemistry showed that the expression of AQP1 in DM group was lower than that in L group, PC group and H group. The expression of AQP1 in L group was lower than that in H group and PC group (P <0.01), but there was no significant difference between H group and PC group P> 0.05). Conclusion: Compound catechu antidiarrheal cream upregulates the expression of AQP1 in colon of diarrhea rats.