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目的:探索125I籽粒间质内放射治疗恶性胶质瘤的合适剂量。方法:将C6胶质瘤株移植到雌性BALB/c无胸腺小鼠右肩皮下建立24只荷瘤鼠模型,并随机分为4组:对照组、空白籽粒组0、.4 mCi(14.8 MBq)籽粒组0、.8 mCi(29.6 MBq)籽粒组。将相应剂量的125I籽粒入肿瘤中心后,观察肿瘤生长情况并绘制肿瘤生长曲线;处死动物后,取肿瘤标本,病理组织切片H-E染色,采用流式细胞术检测细胞凋亡情况,采用免疫组织化学法测定P53蛋白表达。结果:(1)0.4 mCi籽粒组肿瘤体积较对照组及空白籽粒组小(P<0.05);0.8 mCi籽粒组较对照组、空白籽粒组及0.4 mCi籽粒组小(P<0.05);对照组与空白籽粒组无差异(P>0.05);但0.8 mCi籽粒组在种植125I籽粒后3 d内体积较其他3组大(P<0.05)。(2)125I籽粒内照射剂量达到5.8 Gy时开始出现放射性坏死,坏死面积/总面积为(18.33±16.42)%;12 Gy时为(66.67±14.97)%;24 Gy时为(75.83±10.83)%。(3)肿瘤在接受125I24 Gy剂量内照射后凋亡最多;对放射治疗最为敏感的G2-M期细胞在5.8 Gy内照射剂量下最多。(4)肿瘤细胞P53表达在对照组不明显,在3.3 Gy籽粒组和5.8 Gy籽粒组最高。结论:用立体定向技术将小剂量125I籽粒植入脑深部恶性胶质瘤有临床可行性。
Objective: To explore the appropriate dose of 125I interstitial radiation treatment of glioblastoma. METHODS: Twenty-four tumor-bearing mice were established by transplanting C6 glioma into the right shoulder of female BALB / c athymic mice and randomly divided into 4 groups: control group, 0, .4 mCi (14.8 MBq ) Grain group 0, .8 mCi (29.6 MBq) grain group. The corresponding dose of 125I seeds into the tumor center, the growth of the tumor was observed and the tumor growth curve was drawn; after the animals were sacrificed, the tumor samples were taken and pathological sections were stained with HE for the detection of apoptosis by flow cytometry. Immunohistochemistry Method to determine P53 protein expression. Results: (1) The tumor volume of 0.4 mCi seed group was smaller than that of control group and blank seed group (P <0.05); 0.8 mCi grain group was smaller than that of control group, blank grain group and 0.4 mCi grain group (P <0.05) (P> 0.05). However, the volume of the 0.8 mCi grain group was larger than that of the other three groups within 3 d after planting 125I grain (P <0.05). (2) The radioactive necrosis started to occur when the dose of 125I was 5.8 Gy, the area of necrosis / total area was (18.33 ± 16.42)%, (66.67 ± 14.97)% at 12 Gy, (75.83 ± 10.83) at 24 Gy, %. (3) The tumor had the most apoptosis after 125I24 Gy dose irradiation; G2-M phase cells most sensitive to radiation therapy were the most under the irradiation dose of 5.8 Gy. (4) The expression of P53 in tumor cells was not obvious in the control group, which was the highest in 3.3 Gy and 5.8 Gy kernels. Conclusion: The implantation of low doses of 125I seeds into deep brain glioblastoma with stereotactic technique is clinically feasible.