Purpose: To study the antiviral activity of monoclonal antibodies (McAb) in vivo and identify their effects on experimental herpetic keratitis.Methods: Topical use of anti-HSV monoclonal glycoprotein antibodies was carried out on acute herpetic keratitis of rabbits infected by HSV-1 SM44. The application of the eye drops in each group was five times per day for 14 days by double-blind method. In vivo observation and electron microscopy were performed during the whole procedure. The anti-HSV McAb’s solution was mixed up of five monoclonal antibodies with high neutrilization titers and/or high ADCC activity. Results: Compared with placebo-treated eyes, anti-HSV McAb treatment made statistically significant reduction of herpetic corneal epithelial lesion on rabbits from day 3 to day 14 postinnoculation (P<0. 01). Punctate and short dendritic lesion were the main patterns. The area of involvement was also limited. Electron microscopic analysis showed ultrastructural changes of herpetic corneal infection. Th Purpose: To study the antiviral activity of monoclonal antibodies (McAb) in vivo and identify their effects on experimental herpetic keratitis. Methods: Topical use of anti-HSV monoclonal glycoprotein antibodies was carried out on acute herpetic keratitis of rabbits infected by HSV-1 SM44 The application of the eye drops in each group was five times per day for 14 days by double-blind method. The in-vivo observation and electron microscopy were performed during the whole procedure. The anti-HSV McAb’s solution was mixed up five dose with high neutrilization titers and / or high ADCC activity. Results: Compared with placebo-treated eyes, anti-HSV McAb treatment was significantly reduced of herpetic corneal epithelial lesion on rabbits from day 3 to day 14 postinnoculation (P <0.01) Punctate and short dendritic lesion were the main patterns. The area of ​​involvement was also limited. Electron microscopic Analysis showed ultrastructural changes of herpetic cor neal infection. Th