论文部分内容阅读
目的探讨细胞遗传学和免疫细胞化学检查在非霍奇金淋巴瘤骨髓侵犯(NHL-BMI)中的诊断价值。方法骨髓标本直接涂片Wright-Gimsa染色观察其形态学改变。应用骨髓细胞短期培养法制备染色体标本,以R显带技术进行核型分析。分离骨髓单个核细胞进行免疫细胞化学染色。结果24例淋巴瘤患者,骨髓受累程度各异,淋巴瘤细胞形态多样。CD20和CD79a对B细胞来源、CD3对T细胞来源淋巴瘤有诊断价值。有染色体核型异常的病例占54%(13/24),2例骨髓轻度受累患者检出核型异常。结论综合细胞遗传学、免疫细胞化学和细胞形态学检测,有助于NHL骨髓侵润的诊断,特别是对无表浅淋巴结肿大的NHL患者可能提供诊断依据。
Objective To investigate the diagnostic value of cytogenetics and immunocytochemistry in the diagnosis of non-Hodgkin’s lymphoma (NHL-BMI). Methods Wright-Gimsa staining was used to observe the morphological changes of bone marrow specimens. Chromosome specimens were prepared by short-term culture of bone marrow cells and analyzed by R banding. Bone marrow mononuclear cells were isolated for immunocytochemical staining. Results Twenty-four patients with lymphoma had varying degrees of bone marrow involvement and varied lymphoma cells. CD20 and CD79a on B cell source, CD3 on T cell lymphoma have diagnostic value. Chromosome karyotype abnormalities accounted for 54% of cases (13/24), 2 cases of patients with mild bone marrow karyotype abnormalities were detected. Conclusion The comprehensive cytogenetics, immunocytochemistry and cytomorphology detection are helpful to the diagnosis of NHL bone marrow infiltration, especially for patients with NHL without superficial lymph nodes.