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高效液相色谱系统的许多问题都能在谱图上反映出来,一些问题可通过改变设备参数解决,一些问题必须通过修改操作程序解决,而正确选择色谱柱和流动相是得到好的色谱图的关键。一、常见故障及排除1.基线漂移偏大(1)柱温波动:控制好柱子和流动相的温度。(2)流通池被污染或有气泡:用甲醇或其他强极性溶剂冲洗流通池。(3)检测器出现阻塞,高压造成流通池窗口破裂,产生噪音基线:取出阻塞物或更换管子,更换流通池窗需参考检测器手册。(4)柱平衡慢:特别是流动相发生变化时,用中等强度的溶剂冲洗,提前预约客户,在分析前用10~20倍
Many of the problems of HPLC systems can be reflected in the spectra. Some of the problems can be solved by changing the device parameters. Some problems must be solved by modifying the operating procedures. Choosing the correct column and mobile phase is a good chromatogram The essential. A common failure and exclusion 1. Baseline drift is too large (1) Column temperature fluctuations: control the temperature of the column and the mobile phase. (2) The flow cell is contaminated or air bubbles: Flush the flow cell with methanol or other strongly polar solvents. (3) Blocking of the detector, cracking of the flow cell window caused by high pressure, resulting in a noise baseline: remove the obstruction or replace the tube. Refer to the detector manual for changing the flow cell window. (4) column balance slowly: especially when the mobile phase changes, rinse with medium-strength solvent, pre-order customers, before the analysis with 10 to 20 times