论文部分内容阅读
十字花科黑腐病菌(Xanthomonas campestris pv.campertris,简称Xcc)可以引起寄主植物萝卜、白菜、芥菜等十字花科植物的黑腐病,也可以引起非寄主植物辣椒的高敏反应(hypersensitive response,HR)。最近的研究发现,在一些动植物病原细菌中,除蛋白外,小RNA也在致病和HR反应中起关键的作用。但是,到目前为止,小RNA是否在Xcc的致病和HR反应中起重要作用尚不清楚。在前期工作中,我们采用RNA深度测序(RNA-Seq)方法,在Xcc中鉴定了五百多个候选小RNA,但它们的功能还不清楚。在本研究中,我们对小RNA s RXcc52的生物学功能进行了研究,结果发现,小RNA s RXcc52的过量表达株(OE52)在辣椒上引起HR反应的时间比带有一个空的过量表达载体质粒的野生型菌株(WT/p BBad)的时间提前了2 h。这个结果表明,s RXcc52与Xcc的HR反应有关。我们的结果为深入研究小RNA在Xcc的HR反应中的作用打下了基础。
Xanthomonas campestris pv. Campertris (Xcc) can cause black rot in cruciferous plants such as radish, cabbage and mustard, as well as hypersensitive response (HRS) in non-host plants ). Recent studies have found that in some plant and animal pathogenic bacteria, in addition to proteins, small RNAs also play key roles in pathogenicity and HR response. However, it remains unclear whether small RNAs play an important role in the pathogenesis and HR response of Xcc. In our previous work, we identified more than 500 candidate small RNAs in Xcc using the RNA-Seq method, but their function is unclear. In this study, we investigated the biological function of small RNAs RXcc52 and found that the overexpression of RXCC52 overexpression strain (OE52) in small RNAs caused an HR response in pepper longer than that with an empty overexpression vector Wild type strain of plasmid (WT / p BBad) was advanced by 2 h. This result shows that s RXcc52 is related to the HR response of Xcc. Our results lay the foundation for further studies on the role of small RNAs in the HR response of Xcc.