逆转座子是植物基因组的重要成分,对基因组的大小、结构、功能和进化都有重要影响。研究表明多种生物和非生物逆境胁迫可以激活植物逆转座子的转录,但其调控机制和功能还属未知。本研究从稻瘟菌侵染后云南地方水稻品种月亮谷的基因转录谱中筛选出3个逆转座子相关基因,通过RT-PCR分析了逆境胁迫处理对其表达水平的影响。结果表明,稻瘟病菌、水杨酸、2,4-D和NaCl处理水稻苗都能诱导这3个基因的快速转录,表明这3个基因能够同时响应生物逆境和非生物逆境,是研究该类逆转座子表达调控的良好候选基因。通过生物信息学分析还发现,它们在月亮谷基因组中没有发生大的结构变异;且在水稻基因组中都具有与植物抗病相关的旁系同源物,意味着其表达后可能对抗性产生影响,因此,这些基因在逆境和非生物逆境中的表达模式和功能值得关注。 Retrotransposons are an important part of the plant genome and have a major impact on the size, structure, function and evolution of the genome. Studies have shown that a variety of biological and abiotic stresses can activate plants to reverse transposon transcription, but its regulatory mechanisms and functions are still unknown. In this study, three retrotransposon-related genes were screened from the gene transcripts of the moon valley in Yunnan local rice varieties after infection by Magnaporthe grisea. The effects of stress treatment on their expression levels were analyzed by RT-PCR. The results showed that the rice blast induced by Magnaporthe grisea, salicylic acid, 2,4-D and NaCl could induce the rapid transcription of these three genes, indicating that these three genes could respond to both biotic and abiotic stress simultaneously. Reversal of transposon expression regulation of good candidate genes. Bioinformatics analysis also found that they did not occur in the grain structure of the moon Valley large structural variation; and in the rice genome has a plant resistance to disease-related paralogues, which means that its expression may affect the resistance Therefore, the expression patterns and functions of these genes in stress and abiotic stress are worth noting.