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[Zhou ZY, Xu C, Guo QS, et al. Asian J Androl 2004;6: 23-28]目的:研究产后小鼠和大鼠附睾中生殖细胞核因子(GCNF)表达的部位及时间。方法:产后不同时间的附睾切片染色后用间接免疫荧光技术及CarlZeiss共聚焦显微统计数码摄影检测GCNF。结果:小鼠附睾首先在第12d测得GCNF,
[Objective] To study the site and time of germ cell nuclear factor (GCNF) expression in postnatal mouse and rat epididymis. Methods: After epididymal slices were stained at different times of postpartum, indirect immunofluorescence technique and CarlZeiss confocal microscopy digital photography were used to detect GCNF. Results: The mouse epididymis first measured GCNF on the 12th day,