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目的 改进雪旺细胞（SCs）的体外培养条件,以获得足够数量较纯净的雪旺细胞应用于周围神经修复。方法 5-7 d的SD仔鼠坐骨神经,采用混合酶长时间多次消化法分离雪旺细胞,阿糖胞苷（cytosine arabinoside,Ara-C）、G418（geneticin）抑制成纤维细胞生长,氟丝扣林促进雪旺细胞分裂增殖,低浓度胰酶快速消化传代进一步纯化雪旺细胞。结果 使用上述方法可以获得大量的高纯度雪旺细胞,经抗S-100蛋白单抗通过SABC法染色鉴定,雪旺细胞纯度可达到97％以上。结论本文所使用的方法是培养和纯化雪旺细胞较为理想的方法,可以满足目前组织工程神经修复的需要。
Objective To improve the conditions for culturing Schwann cells (SCs) in vitro and to obtain a sufficient number of purified Schwann cells for peripheral nerve repair. METHODS: SD rats were subjected to multiple scintillation digestion with Schwann cells. Cytosine arabinoside (Ara-C) and G418 (geneticin) were used to inhibit the growth of fibroblasts. Buckling forest to promote schwann cell division and proliferation, low concentration of pancreatic enzyme digestion and further purification of Schwann cells. Results A large number of high purity Schwann cells can be obtained by the above method. The purity of Schwann cells can reach more than 97% by SABC staining with anti-S-100 monoclonal antibody. Conclusion The method used in this paper is an ideal method for culturing and purifying Schwann cells, which can meet the needs of current tissue engineering nerve repair.