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以黑龙江主栽品种红丰11为母本,与美国品种Clark杂交,再以红丰11为轮回亲本,对回交后代的芽期和苗期的耐旱性进行筛选。结果获得芽期耐旱导入系44个,采用单项方差分析检测到10个控制芽期耐旱性的QTL;获得苗期耐旱导入系46个,检测到影响苗期叶片相对含水量、叶片持水能力、胁迫期间株高变化量的21个QTL。大多数位点的遗传是相互独立的,只有分布于A1、K、I和H连锁群上的Satt449、Satt499、Satt440和Sat_180位点是在芽期、苗期干旱条件下共同检测到的,表明芽期和苗期的耐旱性存在部分的遗传重叠。以上结果为深入研究大豆耐旱性以及进行分子设计育种以累加芽期苗期重要耐旱QTL奠定了基础。
Hongfeng 11, a main cultivar of Heilongjiang, was used as the female parent to breed with the American variety Clark. Then the Hongfeng 11 was selected as the recurrent parent to screen the drought-tolerance of budding and seedling stage of the backcross. Results A total of 44 drought-tolerant inbred lines were obtained. One-way ANOVA was used to detect 10 QTLs for drought tolerance at seedling stage. 46 drought-tolerant inbred lines were obtained at the seedling stage. Relative leaf water content Water capacity, 21 QTLs for plant height variation during stress. Most of the loci are independent of each other. Only the loci Satt449, Satt499, Satt440 and Sat_180 distributed on linkage groups A1, K, I and H were detected jointly under drought conditions at bud and seedling stage, indicating that There is a partial genetic overlap in drought and seedling stage. The above results provide a basis for further research on drought tolerance of soybean and molecular QTL breeding to accumulate important drought tolerance QTLs at the seedling stage.