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用ＰＣＲ－ＳＳＣＰ分析及ＤＮＡ直接测序法检测了１６例胰腺腺癌的Ｐ５３基因５～８外显子，结果发现３７．５％（６／１６）有Ｐ５３基因点突变，突变分布于外显子５～８，无特殊突变倾向点。６例有Ｐ５３突变者，５例为１个等位基因突变，另１个等位基因丢失；１例则同时含有突变型和野生型等位基因。ＤＮＡ测序显示，突变属于碱基转换者５例，其中Ｇ到Ａ４例，Ａ到Ｇ１例；属于碱基颠换者１例，为Ａ到Ｃ替代；突变点位ＣｐＧ二核工苷酸位者１例。结果提示胰腺腺癌中，Ｐ５３基因的突变是一个较常见的现象，抗癌基因Ｐ５３的失活可能在胰腺腺癌的发生发展中起着一定作用。
The P53 gene exons 5～8 in 16 cases of pancreatic adenocarcinoma were detected by PCR-SSCP analysis and DNA direct sequencing. 37.5% (6/16) of the P53 gene point mutations were found, and mutations were found in exons. 5-8, no special tendency to mutation. Six cases had P53 mutations, 5 cases had one allele mutation and the other allele was lost. One case contained both mutant and wild-type alleles. DNA sequencing showed that the mutation belonged to the base conversion in 5 cases, including G to A4 cases, A to G1 cases; belong to the base transposition in 1 case, which was A to C substitution; the mutation site was CpG dinucleotide nucleotides 1 case. The results suggest that mutation of P53 gene is a common phenomenon in pancreatic adenocarcinoma. The inactivation of anticancer gene P53 may play a role in the development of pancreatic adenocarcinoma.