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目的观察维甲酸换肤液对小鼠光损伤皮肤肿瘤的药理效应和皮肤衰老指标的变化。方法模拟皮肤肿瘤自然的形成过程,应用UVC联合DMBA快速建立小鼠背部皮肤光损伤肿瘤动物模型,2%、1%、0.5%维甲酸换肤液高、中、低剂量分别外涂小鼠背部荷瘤区皮肤,3 min后用生理盐水冲洗局部,终止反应,每天1次,连续3次,14 d后,观察小鼠乳头瘤平均荷瘤数和平均肿瘤面积在治疗前后的改变、皮肤组织形态学变化及皮肤抗衰老指标的测定。结果第12周造模结束时,模型组小鼠平均荷瘤数为(4.57±3.03)个,平均肿瘤体积为(44.9±4.6)mm3。维甲酸高、中、低剂量组涂抹后,均可见瘤体迅速消退,与模型组相比,每鼠平均荷瘤数分别下降为(0.68±0.06)、(2.03±0.17)、(3.03±0.35)个,肿瘤体积分别为(8.3±1.2)、(21.6±1.5)、(29.6±1.4)mm3,抑瘤率分别达到85.1%、55.6%、33.7%,并且换肤液组表皮厚度和真皮胶原合成增加,皮肤羟脯氨酸含量显著增多,皮肤SOD活性增强。结论维甲酸换肤液外用对小鼠光损伤皮肤肿瘤有治疗效果,其疗效与浓度有一定的量效关系。高浓度维甲酸换肤液在抑瘤的同时,亦显示明显的抗皮肤衰老作用。
Objective To observe the pharmacological effects of retinoic acid on skin tumors in mice and the changes of skin aging index. Methods To simulate the natural process of skin tumors, UVC and DMBA were used to establish animal models of photodamage of the back skin of mice. The high, medium and low doses of 2%, 1%, 0.5% Tumor area skin, 3 min after washing with saline local, termination of the reaction, once a day, 3 times in a row, 14 d later, observed the average number of tumor bearing mice and average tumor area of the tumor before and after treatment changes, skin tissue Morphological changes and skin anti-aging index determination. Results At the end of the 12th week, the average number of tumor-bearing mice in model group was (4.57 ± 3.03) and the average tumor volume was (44.9 ± 4.6) mm3. Treated with high, medium and low doses of retinoic acid, the tumors rapidly subsided. Compared with the model group, the average number of tumors in each group decreased (0.68 ± 0.06), (2.03 ± 0.17), (3.03 ± 0.35 ), The tumor volume was (8.3 ± 1.2), (21.6 ± 1.5) and (29.6 ± 1.4) mm3, respectively. The tumor inhibition rates were 85.1%, 55.6% and 33.7%, respectively. The thickness of epidermis and dermal collagen The synthesis increased, the content of hydroxyproline in skin increased significantly, and the activity of SOD in skin increased. Conclusion Retinoic acid skin lotion has therapeutic effect on light-injured skin tumors in mice, and its efficacy and concentration have a certain dose-effect relationship. High concentrations of retinoic acid in the anti-tumor skin, but also showed significant anti-skin aging.