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抑制Ⅳ型胶原酶在肿瘤组织中的过度表达及中和其生物活性是阻止肿瘤新生血管形成和肿瘤转移的手段之一。我们曾报道过抗Ⅳ型胶原酶单链基因工程抗体的构建及其在大肠杆菌中的高效表达。本文将探讨基因工程抗体在Ｐｉｃｈｉａｐａｓｔｏｒｉｓ酵母中的分泌表达。首先构建重组人
Inhibition of type IV collagenase in tumor tissue overexpression and neutralization of its biological activity is to prevent tumor neovascularization and tumor metastasis means. We have reported the construction of an anti-type IV collagenase single chain genetically engineered antibody and its high expression in E. coli. This article examines the secretive expression of engineered antibodies in Pichia pastoris yeast. First build recombinant people