【摘 要】
：
从新鲜牛奶中分离出21株产乳酸球菌,经抑菌试验选出抑菌能力最强菌株SN-21,经鉴定SN-21菌株为乳酸乳球菌乳酸亚种(Lactococcus lactis subsp.lactis).以SN-21为出发菌株,经硫
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从新鲜牛奶中分离出21株产乳酸球菌,经抑菌试验选出抑菌能力最强菌株SN-21,经鉴定SN-21菌株为乳酸乳球菌乳酸亚种(Lactococcus lactis subsp.lactis).以SN-21为出发菌株,经硫酸二乙酯和紫外线诱变处理,获得了一株nisin高产菌株N21(1514.0IU/mL).经发酵条件研究,在蔗糖1﹪,蛋白胨1﹪,牛肉膏0.35﹪,酵母膏0.35﹪,KH<,2>PO<,4>1﹪,NaCl0.2﹪,MgSO<,4>·7H<,2>O0.02﹪,pH6.5的优化条件下N21的nisin效价达到了1862.0IU/mL.
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