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[目的]观察大鼠孕期处于应激状态对其子代情绪的影响,从海马神经递质变化探讨其可能机制。[方法]建立孕期慢性不可预知温和应激大鼠(chronic unpredictable mild stress,CUMS)模型,采用放射免疫法测定母鼠血浆皮质酮水平,利用液体消耗实验、行为学旷场实验、悬尾实验进行子鼠情绪变化的测定,ELISA方法测定子鼠大脑海马组织中单胺类神经递质质量浓度,并分析其相关性。[结果]模型组母鼠皮质酮水平与对照组比较,差异有统计学意义(F=12.347,P=0.001),提示模型组大鼠处于应激状态。模型子鼠组的体重在PND 28和PND 42均低于对照子鼠组(P<0.05);子鼠情绪变化:模型子鼠组水平及垂直运动得分低于对照子鼠组(P<0.05);糖水消耗和1%蔗糖偏爱百分比模型子鼠组得分均低于对照子鼠组(P<0.05);悬尾实验模型子鼠组较对照子鼠组静止时间长、挣扎次数低(P<0.05)。模型子鼠组单胺类神经递质(去甲肾上腺素、多巴胺、5-羟色胺)均下降。[结论]孕期慢性应激影响子鼠情绪变化,可能与母体血浆皮质酮升高,与子鼠海马组织中枢神经递质降低有关。
[Objective] To observe the effect of stress during pregnancy on the offspring emotion of rats and to explore its possible mechanism from the change of neurotransmitter in hippocampus. [Methods] The model of chronic unpredictable mild stress (CUMS) during pregnancy was established. Plasma corticosterone level was measured by radioimmunoassay. The fluid consumption experiment, open field behavior test and tail-suspension experiment Determination of emotional changes in offspring rats, ELISA method for determination of monoamine neurotransmitter mass concentration in the rat brain hippocampus, and analysis of their relevance. [Results] Compared with the control group, the corticosterone level in the model group was significantly different (F = 12.347, P = 0.001), suggesting that the model group rats were in stress state. The body weight of the model group was lower than that of the control group (P <0.05) at PND 28 and PND 42; The level of emotion and the vertical movement of the model group were lower than that of the control group (P <0.05) (P <0.05). Compared with the control group, the mice in the tail suspension model had longer resting time and lower number of struggles than those in the control group (P <0.05) ). The monoamine neurotransmitters (norepinephrine, dopamine, serotonin) decreased in the model group. [Conclusion] Chronic stress during pregnancy may affect the emotional changes of the offspring, which may be related to the increase of plasma corticosterone and the decrease of the neurotransmitter in the hippocampus of the offspring.